Exo-Proofreading, A Versatile SNP Scoring Technology

We report the validation of a new assay for typing single nucleotide polymorphisms (SNPs) that takes advantage of the 3′-to-5′ exonuclease proofreading activity of many DNA polymerases. The assay uses one or more primers labeled on the 3′ nucleotide base, and can be implemented in a variety of forma...

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Bibliografiske detaljer
Main Authors: Cahill, Patrick, Bakis, Michele, Hurley, James, Kamath, Veena, Nielsen, William, Weymouth, Dina, Dupuis, Josée, Doucette-Stamm, Lynn, Smith, Douglas R.
Format: Artigo
Sprog:Inglês
Udgivet: Cold Spring Harbor Laboratory Press 2003
Fag:
Methods
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC430895/
https://ncbi.nlm.nih.gov/pubmed/12695330
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.939903
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