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Gene cloning for the isolation of enzymes of membrane lipid synthesis: phosphatidylserine synthase overproduction in Escherichia coli.

We have screened a bank of 2000 E. coli strains carrying hybrid ColE1 plasmids [Clarke, L. & Carbon, J. (1976) Cell 9, 91-99] for those that correct the temperature sensitivity of a mutant in CDP-1,2-diacyl sn-glycerol:L-serine O-phosphatidyltransferase (EC 2.7.8.8, phosphatidylserine synthase). Two...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Proc Natl Acad Sci U S A
Prif Awduron: Raetz, C R, Larson, T J, Dowhan, W
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: National Academy of Sciences 1977
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC430779/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/193099/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.74.4.1412
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