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Creating Site-Specific Isopeptide Linkages Between Proteins with the Traceless Staudinger Ligation

Site-specific isopeptide linkages between the ε-amino group of a lysine residue in one protein and a carboxyl group in another are central to ubiquitin-mediated protein degradation and other cellular processes. These linkages are inaccessible with common recombinant DNA techniques. Here, we describe...

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Publicado en:Methods Mol Biol
Autores principales: Andersen, Kristen A., Raines, Ronald T.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2015
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4307611/
https://ncbi.nlm.nih.gov/pubmed/25616325
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-2020-4_4
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