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Creating Site-Specific Isopeptide Linkages Between Proteins with the Traceless Staudinger Ligation
Site-specific isopeptide linkages between the ε-amino group of a lysine residue in one protein and a carboxyl group in another are central to ubiquitin-mediated protein degradation and other cellular processes. These linkages are inaccessible with common recombinant DNA techniques. Here, we describe...
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| Publicado no: | Methods Mol Biol |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2015
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4307611/ https://ncbi.nlm.nih.gov/pubmed/25616325 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-4939-2020-4_4 |
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