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Studies on nucleic acid reassociation kinetics: rate of hybridization of excess RNA with DNA, compared to the rate of DNA renaturation.

The rate of reaction of double-stranded replicative form (RF) [3H]DNA of bacteriophage phiX174 with excess (+)strand DNA and (+)strand RNA was measured by standard methods of hydroxyapatite chromatography. The reactions follow pseudo-first-order kinetics and the observed rate constant for the RNA-DN...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Galau, G A, Britten, R J, Davidson, E H
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1977
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC430570/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/265546/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.74.3.1020
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