Identification of Valid Reference Genes for the Normalization of RT-qPCR Expression Studies in Human Breast Cancer Cell Lines Treated with and without Transient Transfection

Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is a powerful technique for examining gene expression changes during tumorigenesis. Target gene expression is generally normalized by a stably expressed endogenous reference gene; however, reference gene expression may differ amo...

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Detalhes bibliográficos
Publicado no:PLoS One
Principais autores: Liu, Lin-Lin, Zhao, Hui, Ma, Teng-Fei, Ge, Fei, Chen, Ce-Shi, Zhang, Ya-Ping
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2015
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4305315/
https://ncbi.nlm.nih.gov/pubmed/25617865
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0117058
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