Protein Structural Studies by Paramagnetic Solid-State NMR Spectroscopy Aided by a Compact Cyclen-Type Cu(II) Binding Tag

Paramagnetic relaxation enhancements (PREs) are a rich source of structural information in protein solid-state NMR spectroscopy. Here we demonstrate that PRE measurements in natively diamagnetic proteins are facilitated by a thiol-reactive compact, cyclen-based, high-affinity Cu(2+) binding tag, 1-(...

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Vydáno v:J Biomol NMR
Hlavní autoři: Sengupta, Ishita, Gao, Min, Arachchige, Rajith J., Nadaud, Philippe S., Cunningham, Timothy F., Saxena, Sunil, Schwieters, Charles D., Jaroniec, Christopher P.
Médium: Artigo
Jazyk:Inglês
Vydáno: 2014
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4304965/
https://ncbi.nlm.nih.gov/pubmed/25432438
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10858-014-9880-9
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