Purification, crystallization and preliminary X-ray diffraction analysis of SpaD, a backbone-pilin subunit encoded by the fimbrial spaFED operon in Lactobacillus rhamnosus GG

SpaD is the predicted backbone-pilin subunit of the SpaFED pilus, whose loci are encoded by the fimbrial spaFED operon in Lactobacillus rhamnosus GG, a Gram-positive gut-adapted commensal strain with perceived probiotic benefits. In this study, soluble recombinant SpaD protein was overproduced in Es...

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Bibliographic Details
Published in:Acta Crystallogr F Struct Biol Commun
Main Authors: Chaurasia, Priyanka, von Ossowski, Ingemar, Palva, Airi, Krishnan, Vengadesan
Format: Artigo
Language:Inglês
Published: International Union of Crystallography 2015
Subjects:
Research Communications
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4304758/
https://ncbi.nlm.nih.gov/pubmed/25615979
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S2053230X14027216
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