An assay of ribonuclease H, endoribonucleases, and phosphatases.
An assay for ribonuclease H (EC 3.1.4.34) is described which permits the estimation of the number and the type of cleavages produced by the enzyme when acting on the ribo moiety of a DNA-RNA hybrid substrate. With six different homopolymer hybrids tested, the number of enzymically induced breaks var...
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| Publicado no: | Proc Natl Acad Sci U S A |
|---|---|
| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1976
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC430336/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/5724/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.73.5.1556 |
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