Assessing Cholesterol Storage in Live Cells and C. elegans by Stimulated Raman Scattering Imaging of Phenyl-Diyne Cholesterol

We report a cholesterol imaging method using rationally synthesized phenyl-diyne cholesterol (PhDY-Chol) and stimulated Raman scattering (SRS) microscope. The phenyl-diyne group is biologically inert and provides a Raman scattering cross section that is 88 times larger than the endogenous C = O stre...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Lee, Hyeon Jeong, Zhang, Wandi, Zhang, Delong, Yang, Yang, Liu, Bin, Barker, Eric L., Buhman, Kimberly K., Slipchenko, Lyudmila V., Dai, Mingji, Cheng, Ji-Xin
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2015
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4302291/
https://ncbi.nlm.nih.gov/pubmed/25608867
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep07930
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