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Synthesis of fluorescent D-amino acids (FDAAs) and their use for probing peptidoglycan synthesis and bacterial growth in situ

Fluorescent D-amino acids (FDAAs) are efficiently incorporated into the peptidoglycan of diverse bacterial species at the sites of active peptidoglycan biosynthesis, allowing specific and covalent probing of bacterial growth with minimal perturbation. Here, we provide a protocol for the synthesis of...

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Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Nat Protoc
Prif Awduron: Kuru, Erkin, Tekkam, Srinivas, Hall, Edward, Brun, Yves V., VanNieuwenhze, Michael S.
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 2014
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC4300143/
https://ncbi.nlm.nih.gov/pubmed/25474031
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2014.197
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