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Synthesis of fluorescent D-amino acids (FDAAs) and their use for probing peptidoglycan synthesis and bacterial growth in situ

Fluorescent D-amino acids (FDAAs) are efficiently incorporated into the peptidoglycan of diverse bacterial species at the sites of active peptidoglycan biosynthesis, allowing specific and covalent probing of bacterial growth with minimal perturbation. Here, we provide a protocol for the synthesis of...

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Bibliografiske detaljer
Udgivet i:Nat Protoc
Main Authors: Kuru, Erkin, Tekkam, Srinivas, Hall, Edward, Brun, Yves V., VanNieuwenhze, Michael S.
Format: Artigo
Sprog:Inglês
Udgivet: 2014
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4300143/
https://ncbi.nlm.nih.gov/pubmed/25474031
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2014.197
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