Synthesis of fluorescent D-amino acids (FDAAs) and their use for probing peptidoglycan synthesis and bacterial growth in situ

Fluorescent D-amino acids (FDAAs) are efficiently incorporated into the peptidoglycan of diverse bacterial species at the sites of active peptidoglycan biosynthesis, allowing specific and covalent probing of bacterial growth with minimal perturbation. Here, we provide a protocol for the synthesis of...

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Detaylı Bibliyografya
Yayımlandı:Nat Protoc
Asıl Yazarlar: Kuru, Erkin, Tekkam, Srinivas, Hall, Edward, Brun, Yves V., VanNieuwenhze, Michael S.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2014
Konular:
Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4300143/
https://ncbi.nlm.nih.gov/pubmed/25474031
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2014.197
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