Accurate Quantification of Episomal HIV-1 Two-Long Terminal Repeat Circles by Use of Optimized DNA Isolation and Droplet Digital PCR

Episomal HIV-1 two-long terminal repeat (2-LTR) circles are considered markers for ongoing viral replication. Two sample processing procedures were compared to accurately quantify 2-LTR in patients by using droplet digital PCR (ddPCR). Here, we show that plasmid isolation with a spiked non-HIV plasm...

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Détails bibliographiques
Publié dans:J Clin Microbiol
Auteurs principaux: Malatinkova, Eva, Kiselinova, Maja, Bonczkowski, Pawel, Trypsteen, Wim, Messiaen, Peter, Vermeire, Jolien, Verhasselt, Bruno, Vervisch, Karen, Vandekerckhove, Linos, De Spiegelaere, Ward
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology 2015
Sujets:
Virology
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4298544/
https://ncbi.nlm.nih.gov/pubmed/25502524
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.03087-14
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