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Preparation and Properties of a Cephalosporin Acetylesterase Adsorbed onto Bentonite
A cephalosporin acetylesterase produced by Bacillus subtilis was immobilized by adsorption onto bentonite. The immobilized enzyme (E(I)) and the soluble enzyme (E(S)) exhibited Michaelis-Menton kinetics with 7-aminocephalosporanic acid (7-ACA): K(m) = 2.8 × 10(−3) M and K(m) = 3.2 × 10(−3) M, respec...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1975
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC429307/ https://ncbi.nlm.nih.gov/pubmed/241288 |
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