Restriction Cascade Exponential Amplification (RCEA) assay with an attomolar detection limit: a novel, highly specific, isothermal alternative to qPCR

An alternative to qPCR was developed for nucleic acid assays, involving signal rather than target amplification. The new technology, Restriction Cascade Exponential Amplification (RCEA), relies on specific cleavage of probe-target hybrids by restriction endonucleases (REase). Two mutant REases for a...

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Publicado no:Sci Rep
Main Authors: Ghindilis, Andrey L., Smith, Maria W., Simon, Holly M., Seoudi, Ihab A., Yazvenko, Nina S., Murray, Iain A., Fu, Xiaoqing, Smith, Kenneth, Jen-Jacobson, Linda, Xu, Shuang-yong
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2015
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4291554/
https://ncbi.nlm.nih.gov/pubmed/25583452
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep07737
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