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The frequency of gene targeting in yeast depends on the number of target copies.

We have compared the efficiency of transformation by linear DNA fragments in yeast strains carrying different numbers of homologous targets for recombination. In strains carrying dispersed copies of a target and in strains carrying tandem arrays, the frequency of transformation is proportional to th...

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Detalhes bibliográficos
Main Authors: Wilson, J H, Leung, W Y, Bosco, G, Dieu, D, Haber, J E
Formato: Artigo
Idioma:Inglês
Publicado em: 1994
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC42909/
https://ncbi.nlm.nih.gov/pubmed/8278360
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