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Nic1 Inactivation Enables Stable Isotope Labeling with (13)C(6)(15)N(4)-Arginine in Schizosaccharomyces pombe

Stable Isotope Labeling by Amino Acids (SILAC) is a commonly used method in quantitative proteomics. Because of compatibility with trypsin digestion, arginine and lysine are the most widely used amino acids for SILAC labeling. We observed that Schizosaccharomyces pombe (fission yeast) cannot be labe...

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Detalhes bibliográficos
Publicado no:Mol Cell Proteomics
Main Authors: Carpy, Alejandro, Patel, Avinash, Tay, Ye Dee, Hagan, Iain M., Macek, Boris
Formato: Artigo
Idioma:Inglês
Publicado em: The American Society for Biochemistry and Molecular Biology 2015
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4288259/
https://ncbi.nlm.nih.gov/pubmed/25368411
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.O114.045302
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