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Double trouble—Buffer selection and His-tag presence may be responsible for nonreproducibility of biomedical experiments
The availability of purified and active protein is the starting point for the majority of in vitro biomedical, biochemical, and drug discovery experiments. The use of polyhistidine affinity tags has resulted in great increases of the efficiency of the protein purification process, but can negatively...
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| Publicado no: | Protein Sci |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BlackWell Publishing Ltd
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4286991/ https://ncbi.nlm.nih.gov/pubmed/25044180 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.2520 |
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