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Site-directed mutagenesis in Arabidopsis thaliana using dividing tissue-targeted RGEN of the CRISPR/Cas system to generate heritable null alleles

MAIN CONCLUSION: Dividing tissue-targeted site-directed mutagenesis using RGEN of CRISPR/Cas system produces heritable mutations in Arabidopsis thaliana. ABSTRACT: Site-directed genome engineering in higher plants has great potential for basic research and molecular breeding. Here, we describe a met...

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Detalhes bibliográficos
Publicado no:Planta
Main Authors: Hyun, Youbong, Kim, Jungeun, Cho, Seung Woo, Choi, Yeonhee, Kim, Jin-Soo, Coupland, George
Formato: Artigo
Idioma:Inglês
Publicado em: Springer Berlin Heidelberg 2014
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4282705/
https://ncbi.nlm.nih.gov/pubmed/25269397
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00425-014-2180-5
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