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Cytosine deaminase as a negative selectable marker for the microalgal chloroplast: a strategy for the isolation of nuclear mutations that affect chloroplast gene expression

Negative selectable markers are useful tools for forward-genetic screens aimed at identifying trans-acting factors that are required for expression of specific genes. Transgenic lines harbouring the marker fused to a gene element, such as a promoter, may be mutagenized to isolate loss-of-function mu...

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Publicat a:Plant J
Autors principals: Young, Rosanna E B, Purton, Saul
Format: Artigo
Idioma:Inglês
Publicat: BlackWell Publishing Ltd 2014
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC4282525/
https://ncbi.nlm.nih.gov/pubmed/25234691
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/tpj.12675
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