Integration of Host Strain Bioengineering and Bioprocess Development Using Ultra-Scale Down Studies to Select the Optimum Combination: An Antibody Fragment Primary Recovery Case Study

An ultra scale-down primary recovery sequence was established for a platform E. coli Fab production process. It was used to evaluate the process robustness of various bioengineered strains. Centrifugal discharge in the initial dewatering stage was determined to be the major cause of cell breakage. T...

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Bibliographic Details
Published in:Biotechnol Bioeng
Main Authors: Aucamp, Jean P, Davies, Richard, Hallet, Damien, Weiss, Amanda, Titchener-Hooker, Nigel J
Format: Artigo
Language:Inglês
Published: BlackWell Publishing Ltd 2014
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4282095/
https://ncbi.nlm.nih.gov/pubmed/24838387
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/bit.25259
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