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Effects of temperature and glycerol and methanol-feeding profiles on the production of recombinant galactose oxidase in Pichia pastoris

Optimization of protein production from methanol-induced Pichia pastoris cultures is necessary to ensure high productivity rates and high yields of recombinant proteins. We investigated the effects of temperature and different linear or exponential methanol-feeding rates on the production of recombi...

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Bibliografiske detaljer
Udgivet i:Biotechnol Prog
Main Authors: Anasontzis, George E, Salazar Penã, Margarita, Spadiut, Oliver, Brumer, Harry, Olsson, Lisbeth
Format: Artigo
Sprog:Inglês
Udgivet: BlackWell Publishing Ltd 2014
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4282061/
https://ncbi.nlm.nih.gov/pubmed/24493559
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/btpr.1878
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