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Culturing of respiratory viruses in well-differentiated pseudostratified human airway epithelium as a tool to detect unknown viruses

BACKGROUND: Currently, virus discovery is mainly based on molecular techniques. Here, we propose a method that relies on virus culturing combined with state-of-the-art sequencing techniques. The most natural ex vivo culture system was used to enable replication of respiratory viruses. METHOD: Three...

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Publicado en:Influenza Other Respir Viruses
Autores principales: Jazaeri Farsani, Seyed Mohammad, Deijs, Martin, Dijkman, Ronald, Molenkamp, Richard, Jeeninga, Rienk E, Ieven, Margareta, Goossens, Herman, van der Hoek, Lia
Formato: Artigo
Lenguaje:Inglês
Publicado: Blackwell Publishing Ltd 2015
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC4280819/
https://ncbi.nlm.nih.gov/pubmed/25482367
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/irv.12297
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