Suitable housekeeping genes for normalization of transcript abundance analysis by real-time RT-PCR in cultured bovine granulosa cells during hypoxia and differential cell plating density

BACKGROUND: Bovine granulosa cell culture models are important to understand molecular mechanisms of ovarian function. Folliculogenesis and luteinization are associated with increasing density of cells and local hypoxic conditions. The current study identified two reliable housekeeping genes useful...

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Detalhes bibliográficos
Publicado no:Reprod Biol Endocrinol
Main Authors: Baddela, Vijay S, Baufeld, Anja, Yenuganti, Vengala R, Vanselow, Jens, Singh, Dheer
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2014
Assuntos:
Methodology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4280684/
https://ncbi.nlm.nih.gov/pubmed/25430436
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1477-7827-12-118
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