Saturation Mutagenesis of Toluene ortho-Monooxygenase of Burkholderia cepacia G4 for Enhanced 1-Naphthol Synthesis and Chloroform Degradation

Directed evolution of toluene ortho-monooxygenase (TOM) of Burkholderia cepacia G4 previously created the hydroxylase α-subunit (TomA3) V106A variant (TOM-Green) with increased activity for both trichloroethylene degradation (twofold enhancement) and naphthalene oxidation (six-times-higher activity)...

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Veröffentlicht in:	Appl Environ Microbiol
Hauptverfasser:	Rui, Lingyun, Kwon, Young Man, Fishman, Ayelet, Reardon, Kenneth F., Wood, Thomas K.
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 2004
Schlagworte:	Enzymology and Protein Engineering
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC427800/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15184118/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.70.6.3246-3252.2004
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