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Facile and high-efficient immobilization of histidine-tagged multimeric protein G on magnetic nanoparticles
This work reports the high-efficient and one-step immobilization of multimeric protein G on magnetic nanoparticles. The histidine-tagged (His-tag) recombinant multimeric protein G was overexpressed in Escherichia coli BL21 by the repeated linking of protein G monomers with a flexible linker. High-ef...
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| Publicado no: | Nanoscale Res Lett |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Springer
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4277612/ https://ncbi.nlm.nih.gov/pubmed/25593554 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1556-276X-9-664 |
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