Purification of Phytochrome by Affinity Chromatography on Agarose-Immobilized Cibacron Blue 3GA
The binding of phytochrome to Cibacron Blue 3GA was utilized to develop a new affinity purification procedure for phytochrome. Brushite-purified phytochrome from rye (Secale cereale c.v. Cougar) was bound to agarose-immobilized blue dye in 0.1 molar potassium phosphate (pH 7.8), contaminating protei...
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| Publicado no: | Plant Physiol |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
1981
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC427507/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16661933/ https://ncbi.nlm.nih.govhttps://doi.org/10.1104/pp.68.2.443 |
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