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3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
The objective of this video protocol is to discuss how to perform and analyze a three-dimensional fluorescent orbital particle tracking experiment using a modified two-photon microscope(1). As opposed to conventional approaches (raster scan or wide field based on a stack of frames), the 3D orbital t...
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| Publicado no: | J Vis Exp |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
MyJove Corporation
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4274936/ https://ncbi.nlm.nih.gov/pubmed/25350070 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51794 |
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