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Distribution of Human-Specific Bacteroidales and Fecal Indicator Bacteria in an Urban Watershed Impacted by Sewage Pollution, Determined Using RNA- and DNA-Based Quantitative PCR Assays

The identification of fecal pollution sources is commonly carried out using DNA-based methods. However, there is evidence that DNA can be associated with dead cells or present as “naked DNA” in the environment. Furthermore, it has been shown that rRNA-targeted reverse transcription-quantitative PCR...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Main Authors: Kapoor, Vikram, Pitkänen, Tarja, Ryu, Hodon, Elk, Michael, Wendell, David, Santo Domingo, Jorge W.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2014
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4272702/
https://ncbi.nlm.nih.gov/pubmed/25326295
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02446-14
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