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Preparation of Soluble Proteins from Escherichia coli
Purification of human IL-1β is used in this unit as an example of the preparation of soluble proteins from E. coli. Bacteria containing IL-1β are lysed, and IL-1 β in the resulting supernatant is purified by anion-exchange chromatography, salt precipitation and cation-exchange chromatography, and th...
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| Publicado no: | Curr Protoc Protein Sci |
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| Autor principal: | |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4272062/ https://ncbi.nlm.nih.gov/pubmed/25367009 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/0471140864.ps0602s78 |
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