Substrate Binding to an Active Creatine Kinase with a Thiol-Bound Mercurinitrophenol Chromophoric Probe
A mol of creatine kinase (EC 2.7.3.2), an enzyme with two identical subunits can be titrated very rapidly and stoichiometrically with 2 mol of 2-chloromercuri-4-nitrophenol, an environmentally sensitive chromophoric probe specific for thiols. Chemical modification does not inactivate the enzyme nor...
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| Pubblicato in: | Proc Natl Acad Sci U S A |
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| Autori principali: | , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
National Academy of Sciences
1973
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC427125/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4517940/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.70.10.2858 |
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