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Improvement of Shotgun Proteomics in the Negative Mode by Carbamylation of Peptides and Ultraviolet Photodissociation Mass Spectrometry

[Image: see text] Although acidic peptides compose a substantial portion of many proteomes, their less efficient ionization during positive polarity electrospray ionization (ESI) impedes their detection in bottom-up mass spectrometry workflows. We have implemented a derivatization strategy based on...

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Udgivet i:Anal Chem
Main Authors: Greer, Sylvester M., Cannon, Joe R., Brodbelt, Jennifer S.
Format: Artigo
Sprog:Inglês
Udgivet: American Chemical Society 2014
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4270407/
https://ncbi.nlm.nih.gov/pubmed/25420043
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac5035314
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