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An Oligonucleotide Affinity Column for RNA-Dependent DNA Polymerase from RNA Tumor Viruses

Columns of (dT)(12-18)-cellulose provide a one-step enrichment procedure for RNA-dependent DNA polymerase. The enzyme of the virus from RD-114 cells, as well as that from Rauscher murine leukemia virus, have been purified in this way. The preference of viral as compared to cellular DNA polymerases f...

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Bibliographic Details
Main Authors: Gerwin, Brenda I., Milstien, Julie B.
Format: Artigo
Language:Inglês
Published: 1972
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC426997/
https://ncbi.nlm.nih.gov/pubmed/4506781
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