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Purification of Biologically Active Globin Messenger RNA by Chromatography on Oligothymidylic acid-Cellulose

A convenient technique for the partial purification of large quantities of functional, poly(adenylic acid)-rich mRNA is described. The method depends upon annealing poly(adenylic acid)-rich mRNA to oligothymidylic acid-cellulose columns and its elution with buffers of low ionic strength. Biologicall...

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Detalles Bibliográficos
Publicado en:Proc Natl Acad Sci U S A
Main Authors: Aviv, Haim, Leder, Philip
Formato: Artigo
Idioma:Inglês
Publicado: National Academy of Sciences 1972
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Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC426713/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4504350/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.69.6.1408
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