Nitrogen Ligands at the Active Site of Alkaline Phosphatase
The two Zn(II) ions of native Escherichia coli alkaline phosphatase (EC 3.1.3.1) that are necessary for activity have been replaced by (63)Cu(II). Titration of apoenzyme with up to 2 eq of Cu(II) gives a homogeneous species with an electron spin resonance typical for Cu(II) in an axially symmetric e...
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| Veröffentlicht in: | Proc Natl Acad Sci U S A |
|---|---|
| Hauptverfasser: | , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
National Academy of Sciences
1972
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| Schlagworte: | |
| Online-Zugang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC426581/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4337243/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.69.4.859 |
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