REAL-Select: Full-Length Antibody Display and Library Screening by Surface Capture on Yeast Cells

We describe a novel approach named REAL-Select for the non-covalent display of IgG-molecules on the surface of yeast cells for the purpose of antibody engineering and selection. It relies on the capture of secreted native full-length antibodies on the cell surface via binding to an externally immobi...

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發表在:PLoS One
Main Authors: Rhiel, Laura, Krah, Simon, Günther, Ralf, Becker, Stefan, Kolmar, Harald, Hock, Björn
格式: Artigo
語言:Inglês
出版: Public Library of Science 2014
主題:
Research Article
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4264850/
https://ncbi.nlm.nih.gov/pubmed/25501029
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0114887
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