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Detection of DNA Sequences Refractory to PCR Amplification Using a Biophysical SERRS Assay (Surface Enhanced Resonant Raman Spectroscopy)

The analysis of ancient or processed DNA samples is often a great challenge, because traditional Polymerase Chain Reaction – based amplification is impeded by DNA damage. Blocking lesions such as abasic sites are known to block the bypass of DNA polymerases, thus stopping primer elongation. In the p...

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Bibliografische gegevens
Gepubliceerd in:PLoS One
Hoofdauteurs: Feuillie, Cécile, Merheb, Maxime M., Gillet, Benjamin, Montagnac, Gilles, Daniel, Isabelle, Hänni, Catherine
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Public Library of Science 2014
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4264738/
https://ncbi.nlm.nih.gov/pubmed/25502338
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0114148
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