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Isolation and Characterization of Metabolically Competent Mitochondria from Spinach Leaf Protoplasts

Intact mitochondria were prepared from spinach (Spinacia oleracea L. var. Kyoho) leaf protoplasts and purified by Percoll discontinuous gradient centrifugation. Assays of several marker enzymes showed that the final mitochondrial preparations obtained are nearly free from other contaminating organel...

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Detalhes bibliográficos
Main Authors: Nishimura, Mikio, Douce, Roland, Akazawa, Takashi
Formato: Artigo
Idioma:Inglês
Publicado em: 1982
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC426328/
https://ncbi.nlm.nih.gov/pubmed/16662319
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