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Cryopreservation of Human Pluripotent Stem Cells in Defined Medium
This protocol describes a cryopreservation procedure using an enzyme-free dissociation method to harvest cells and preserve cells in albumin-free chemically defined E8 medium for human pluripotent stem cells (hPSCs). The dissociation by EDTA/PBS produces small cell aggregates that allow high surviva...
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| Published in: | Curr Protoc Stem Cell Biol |
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| Main Authors: | , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
2014
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4252050/ https://ncbi.nlm.nih.gov/pubmed/25366897 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/9780470151808.sc01c17s31 |
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