High-resolution structure of a Shigella type III secretion needle by solid-state NMR and cryo-electron microscopy

We introduce a general hybrid approach for determining the structures of supramolecular assemblies. Cryo-electron microscopy (cryo-EM) data define the overall envelope of the assembly and rigid-body orientation of the subunits while solid-state NMR (ssNMR) chemical shifts and distance constraints de...

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Pubblicato in:Nat Commun
Autori principali: Demers, Jean-Philippe, Habenstein, Birgit, Loquet, Antoine, Vasa, Suresh Kumar, Giller, Karin, Becker, Stefan, Baker, David, Lange, Adam, Sgourakis, Nikolaos G.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2014
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4251803/
https://ncbi.nlm.nih.gov/pubmed/25264107
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms5976
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