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Maintenance of an extrachromosomal plasmid vector in mouse embryonic stem cells.

We have constructed and characterized a polyoma virus-based plasmid that is maintained as an autonomously replicating extrachromosomal element (episome) in mouse embryonic stem (ES) cells. Plasmid pMGD20neo contains the polyoma origin of replication harboring a mutated enhancer (PyF101), a modified...

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Bibliografiske detaljer
Main Authors: Gassmann, M, Donoho, G, Berg, P
Format: Artigo
Sprog:Inglês
Udgivet: 1995
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC42505/
https://ncbi.nlm.nih.gov/pubmed/7877970
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