Maintenance of an extrachromosomal plasmid vector in mouse embryonic stem cells.
We have constructed and characterized a polyoma virus-based plasmid that is maintained as an autonomously replicating extrachromosomal element (episome) in mouse embryonic stem (ES) cells. Plasmid pMGD20neo contains the polyoma origin of replication harboring a mutated enhancer (PyF101), a modified...
Enregistré dans:
| Publié dans: | Proc Natl Acad Sci U S A |
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| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
National Academy of Sciences
1995
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC42505/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7877970/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.92.5.1292 |
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