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Derivation and Long-Term Culture of Transgene-Free Human Induced Pluripotent Stem Cells on Synthetic Substrates

We describe a platform to derive, culture, and differentiate genomically stable, transgene-free human induced pluripotent stem cells (iPSCs) on a fully synthetic polymer substrate made of a grafted zwitterionic hydrogel: poly2-(methacryloyloxy)ethyl dimethyl-(3-sulfopropyl) ammonium hydroxide (PMEDS...

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Detalhes bibliográficos
Publicado no:Stem Cells Transl Med
Main Authors: Villa-Diaz, Luis Gerardo, Kim, Jin Koo, Lahann, Joerg, Krebsbach, Paul H.
Formato: Artigo
Idioma:Inglês
Publicado em: AlphaMed Press 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4250210/
https://ncbi.nlm.nih.gov/pubmed/25313201
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.5966/sctm.2014-0087
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