Sensitive, Efficient Quantitation of (13)C-Enriched Nucleic Acids via Ultrahigh-Performance Liquid Chromatography–Tandem Mass Spectrometry for Applications in Stable Isotope Probing

Stable isotope probing (SIP) of nucleic acids is a powerful tool for studying the functional traits of microbial populations within complex communities, but SIP involves a number of technical challenges. Many of the difficulties in DNA-SIP and RNA-SIP experiments can be effectively overcome with an...

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Veröffentlicht in:Appl Environ Microbiol
Hauptverfasser: Wilhelm, Roland, Szeitz, András, Klassen, Tara L., Mohn, William W.
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology 2014
Schlagworte:
Methods
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4249172/
https://ncbi.nlm.nih.gov/pubmed/25217022
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02223-14
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