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UV-induced Spectral Shift and Protonation of DNA Fluorescent Dye Hoechst 33258

DNA-bound Hoechst 33258 is readily excited with UV light and emits blue fluorescence, however, upon exposure to UV, the dye undergoes photobleaching as well as photoconversion to a blue-excited green-emitting form. We demonstrate that the UV-generated green-emitting form of Hoechst 33258 exhibits sp...

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Detalhes bibliográficos
Publicado no:J Fluoresc
Main Authors: Żurek-Biesiada, Dominika, Waligórski, Piotr, Dobrucki, Jurek W.
Formato: Artigo
Idioma:Inglês
Publicado em: Springer US 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4246133/
https://ncbi.nlm.nih.gov/pubmed/25312832
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10895-014-1468-y
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