Validation of a multiplex reverse transcription and pre-amplification method using TaqMan(®) MicroRNA assays

Since the discovery of microRNAs (miRNAs), different approaches have been developed to label, amplify and quantify miRNAs. The TaqMan(®) technology, provided by Applied Biosystems (ABIs), uses a stem-loop reverse transcription primer system to reverse transcribe the RNA and amplify the cDNA. This me...

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Bibliografische gegevens
Gepubliceerd in:Front Genet
Hoofdauteurs: Le Carré, Joane, Lamon, Séverine, Léger, Bertrand
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Frontiers Media S.A. 2014
Onderwerpen:
Genetics
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4244598/
https://ncbi.nlm.nih.gov/pubmed/25505484
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fgene.2014.00413
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