A method for probing the topography and interactions of proteins: footprinting of myoglobin.
We describe a procedure for mapping residues on the surface of a protein molecule to its sequence, using a scheme that is analogous to nucleic acid footprinting. The protein is end labeled radioactively and subjected to limited proteolysis, and the products are analyzed by denaturing polyacrylamide...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1995
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC42433/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7892233/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.92.6.2111 |
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