The Generation of Successive Unmarked Mutations and Chromosomal Insertion of Heterologous Genes in Actinobacillus pleuropneumoniae Using Natural Transformation

We have developed a simple method of generating scarless, unmarked mutations in Actinobacillus pleuropneumoniae by exploiting the ability of this bacterium to undergo natural transformation, and with no need to introduce plasmids encoding recombinases or resolvases. This method involves two successi...

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Udgivet i:PLoS One
Main Authors: Bossé, Janine T., Soares-Bazzolli, Denise M., Li, Yanwen, Wren, Brendan W., Tucker, Alexander W., Maskell, Duncan J., Rycroft, Andrew N., Langford, Paul R.
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2014
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4237320/
https://ncbi.nlm.nih.gov/pubmed/25409017
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0111252
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