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Oligoribonucleotide (ORN) Interference-PCR (ORNi-PCR): A Simple Method for Suppressing PCR Amplification of Specific DNA Sequences Using ORNs

Polymerase chain reaction (PCR) amplification of multiple templates using common primers is used in a wide variety of molecular biological techniques. However, abundant templates sometimes obscure the amplification of minor species containing the same primer sequences. To overcome this challenge, we...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Tanigawa, Naoki, Fujita, Toshitsugu, Fujii, Hodaka
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2014
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4236179/
https://ncbi.nlm.nih.gov/pubmed/25405983
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0113345
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