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Comparison of in-gel protein separation techniques commonly used for fractionation in mass spectrometry-based proteomic profiling

Fractionation of complex samples at the cellular, subcellular, protein or peptide level is an indispensable strategy to improve the sensitivity in mass spectrometry-based proteomic profiling. This study revisits, evaluates, and compares the most common gel-based protein separation techniques i.e., 1...

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Publicado en:Electrophoresis
Main Authors: Jafari, Mohieddin, Primo, Vincent, Smejkal, Gary B., Moskovets, Eugene V., Kuo, Winston P., Ivanov, Alexander R.
Formato: Artigo
Idioma:Inglês
Publicado: 2012
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4234072/
https://ncbi.nlm.nih.gov/pubmed/22899259
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/elps.201200031
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