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DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems

In vivo methods such as ChIP-chip are well-established techniques used to determine global gene targets for transcription factors. However, they are of limited use in exploring bacterial two component regulatory systems with uncharacterized activation conditions. Such systems regulate transcription...

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Detalles Bibliográficos
Publicado en:J Vis Exp
Main Authors: Rajeev, Lara, Luning, Eric G., Mukhopadhyay, Aindrila
Formato: Artigo
Idioma:Inglês
Publicado: MyJove Corporation 2014
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4233932/
https://ncbi.nlm.nih.gov/pubmed/25079303
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/51715
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