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A practical guide to the synthesis and use of membrane-permeant acetoxymethyl esters of caged inositol polyphosphates
This protocol describes a method for efficient chemical synthesis of an analog of inositol-1,4,5-trisphosphate (IP(3)) hexakis acetoxymethyl ester having an ortho-nitroveratryl photochemical caging group on the 6-hydroxyl position. The six esters render the probe membrane permeant, such that it can...
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| Pubblicato in: | Nat Protoc |
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| Autori principali: | , , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
2011
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4233651/ https://ncbi.nlm.nih.gov/pubmed/21372813 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2010.194 |
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