A Simplified and Versatile System for the Simultaneous Expression of Multiple siRNAs in Mammalian Cells Using Gibson DNA Assembly

RNA interference (RNAi) denotes sequence-specific mRNA degradation induced by short interfering double-stranded RNA (siRNA) and has become a revolutionary tool for functional annotation of mammalian genes, as well as for development of novel therapeutics. The practical applications of RNAi are usual...

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Veröffentlicht in:PLoS One
Hauptverfasser: Deng, Fang, Chen, Xiang, Liao, Zhan, Yan, Zhengjian, Wang, Zhongliang, Deng, Youlin, Zhang, Qian, Zhang, Zhonglin, Ye, Jixing, Qiao, Min, Li, Ruifang, Denduluri, Sahitya, Wang, Jing, Wei, Qiang, Li, Melissa, Geng, Nisha, Zhao, Lianggong, Zhou, Guolin, Zhang, Penghui, Luu, Hue H., Haydon, Rex C., Reid, Russell R., Yang, Tian, He, Tong-Chuan
Format: Artigo
Sprache:Inglês
Veröffentlicht: Public Library of Science 2014
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Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4232585/
https://ncbi.nlm.nih.gov/pubmed/25398142
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0113064
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